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KMID : 1007520090180020561
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Food Science and Biotechnology
2009 Volume.18 No. 2 p.561 ~ p.564
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Determination of 11 Ginsenosides in Black Ginseng Developed from Panax ginseng by High Performance Liquid Chromatography
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Sun Bai-Shen
Gu Li-Juan
Fang Zhe-Ming
Wang Chun-Yan
Wang Zhen
Sung Chang-Keun
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Abstract
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A high performance liquid chromatography (HPLC) method has been developed for determination of 11ginsenosides in black ginseng (BG, white ginseng that is subjected to 9 cycles of 95oC for 3 hr). After eluted by gradientelution of water-acetonitrile without buffer in 70 min, 11 ginsenosides in BG were identified. The proposed method providedgood linearity (R2>0.9995), accuracy (92.2-106.6%), and intra- and interday precision (RSD<2.6%). In addition, ginsenosidescompositions in white, red, and black ginsengs were investigated using this method, respectively. Interestingly, in BG, thecontent of ginsenoside Rg3 which does not existed in white ginseng was 7.51 mg/g, approximately 20 times than that in redginseng.
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KEYWORD
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black ginseng, ginsenoside, high performance liquid chromatography
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